Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures
نویسندگان
چکیده
منابع مشابه
Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures
Embryonic midbrain micromass cultures were exposed for five days to ochratoxin A (OTA) at seven concentrations (ranging from 0.16 to 10 microg/mL). Cell viability was assessed in neutral red uptake test (NRU), and differentiation - by immunoenzymatic determination of structural proteins (beta(III)-tubulin, MAP2, GFAP) expression level as well as by computer image analysis. Dose dependent decrea...
متن کاملEmbryotoxicity of Carbendazim in Rat and Hamster Micromass Cultures
The embryotoxic potential of the commonly used fungicide, methyl 2-benzimidazolecarbamate (MBC) was evaluated in rat and hamster embryo limb bud (LB) and midbrain (CNS) cell micromass cultures. Cultures were exposed to seven concentrations of 0.03, 0.12, 0.49, 1.95, 7.81, 31.25 and 125 μg MBC/ml. Developmental toxicity was assessed via the following parameters: cytotoxicity, by quantifying neut...
متن کاملMetabolism of ochratoxin A by primary cultures of rat hepatocytes.
Association of ochratoxin A with cultured rat hepatocytes occurs at 4 degrees C, and the saturation level in the medium is 0.3 mM ochratoxin A, with maximal binding after 60 min. At 37 degrees C the level of cell-associated ochratoxin A increased up to 6 h and remained at 2 nmol of toxin per mg of cell protein for 30 h. With increasing concentrations of ochratoxin A, increasing amounts of the t...
متن کاملEmbryo and Developmental Toxicity of Cefazolin Sodium Impurities in Zebrafish
Cefazolin sodium is an essential drug that is widely used in clinical therapy for certain infective diseases caused by bacteria. As drug impurities are considered to be one of the most important causes of drug safety issues, we studied embryotoxicity, cardiotoxicity, and neurotoxicity of nine cefazolin sodium impurities in zebrafish embryo and larvae for the objective control of impurity profil...
متن کاملIdentification of Teratogens in Cell Culture. Ii. Laboratory Values of Rat Embryo Midbrain Studied by Micromass Test with Penicillin G and 5-fluorouracil
One of the model system that is included in the battery of tests for in vitro embryotoxicity studies is Micromass test. However, this test is based on the rat embryo limb bud cell culture and disregards other tissues. The aim of this work was to optimize culture condition and to check the influence of initial cell density and spot volume plated on the evaluation of embryotoxicity. Two model com...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2008
ISSN: 1422-0067
DOI: 10.3390/ijms10010037